Supporting Figure Legends
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چکیده
Figure 6. Example scatter plots showing binding of p65 and p50 to gene promoters in living cells. Microarray data from p65 ChIP in unstimulated cells and p50 ChIP following LPS stimulation. The x-axis represents the hybridization signal intensity for input whole cell extract (WCE) DNA (log10 Cy3 background-subtracted signal). The yaxis shows the intensity for DNA enriched by ChIP using antibodies specific for p65 or p50 (log10 Cy5 background-subtracted signal). Microarray elements that were flagged as not found by the Axon software were not analyzed. The red lines either side of the scatter plots represent the ratio of IP signal to WCE signal necessary to achieve a significance score of 0.002. The position of the promoters for the known NF-κB target genes HLA-B, NFKB2, CCL1, PTX3, CSF2 and RAC2, the novel NF-κB target genes NFKBIB and P4HA1 and β-actin, a negative control, are marked in both scatter plots. No genes were enriched above the pvalue threshold of 0.002 by p65 ChIP in unstimulated cells. In contrast, HLA-B, NFKB2, CCL1, PTX3, CSF2, RAC2, NFKBIB and P4HA1 were among the 193 genes enriched above this threshold by p50 ChIP in stimulated cells. β-actin was not enriched in either p65 or p50 ChIP.
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تاریخ انتشار 2006